The Automated Optical Microscopy service of the Microscopy and Image Analysis Core has an ImageXpress Micro Confocal High-Content Imaging System for microscopy in multi-well plates. The system has both widefield and confocal modalities and has environmental controls enabling both fixed and live-cell imaging of whole organisms, thick tissues, 3D spheroid assays, and cellular or intracellular events. This instrument along with our liquid dispensers, plate washer and fully customizable analysis software can be employed to automate many types of fluorescence imaging experiments reducing variability and saving hours of operator time.